Journal: Asian Journal of Andrology

Article Title: Energy restriction and exercise modulate angiopoietins and vascular endothelial growth factor expression in the cavernous tissue of high-fat diet-fed rats

doi: 10.1038/aja.2011.131

Figure Lengend Snippet: Dual immunofluorescence labelling of VEGF (red)/VEGFR1 (green) in the rat cavernous tissue of all experimental groups. DAPI (nuclear staining), blue; C, control animal group; ER, energy-restricted animal group; EREx, energy-restricted and exercised animal group; HFD, high-fat fed animal group. Scale bar=100 µm. DAPI, 4′,6-diamidino-2-phenylindole; VEGF, vascular endothelial growth factor; VEGFR, vascular endothelial growth factor receptor.

Article Snippet: Sections were incubated overnight at 4 °C with a mixture of primary antibodies: goat anti-VEGF (R&D Systems, Minneapolis, MN, USA) with rabbit anti-VEGFR1 (Lab Vision Corporation, Fremont, CA, USA) or with mouse anti-VEGFR2 (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA); and rabbit anti-Tie2 with goat anti-Ang1 or with goat anti-Ang2 (Santa Cruz Biotechnology).

Techniques: Immunofluorescence, Staining

Journal: Asian Journal of Andrology

Article Title: Energy restriction and exercise modulate angiopoietins and vascular endothelial growth factor expression in the cavernous tissue of high-fat diet-fed rats

doi: 10.1038/aja.2011.131

Figure Lengend Snippet: Western blot analysis of VEGF, VEGFR1, VEGFR2, Ang1, Ang2 and Tie2. Representative bands obtained by Western blot analysis of VEGF (a), VEGFR1 (b), VEGFR2 (c), Ang1 (d), Ang2 (e) and Tie2 (f), in rat cavernous tissue of all experimental groups. The graphs below represent the semiquantitative analysis of the aforementioned proteins (n=4 for each group), calculated by the fraction of pixels presented by each band relative to β-actin band used as the loading control. Error bars represent standard deviation. C, control animal group; ER, energy-restricted animal group; EREx, energy-restricted and exercised animal group; HFD, high-fat diet-fed animal group. *P<0.05 between HFD and the corresponding 8- and 16-week control groups (black bars); †P<0.05 between ER or EREx, and 16-week HFD group; *†P<0.05 between ER or EREx, and the 16-week control and HFD groups. Ang, angiopoietin; VEGF, vascular endothelial growth factor; VEGFR, vascular endothelial growth factor receptor.

Article Snippet: Sections were incubated overnight at 4 °C with a mixture of primary antibodies: goat anti-VEGF (R&D Systems, Minneapolis, MN, USA) with rabbit anti-VEGFR1 (Lab Vision Corporation, Fremont, CA, USA) or with mouse anti-VEGFR2 (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA); and rabbit anti-Tie2 with goat anti-Ang1 or with goat anti-Ang2 (Santa Cruz Biotechnology).

Techniques: Western Blot, Standard Deviation